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Schlaeppi, K.; Bender, S.F.; Mascher, F.; Russo, G.; Patrignani, A.; Camenzind, T.; Hempel, S.; Rillig, M.C. & van der Heijden, M.G. (2016): High-resolution community profiling of arbuscular mycorrhizal fungi. New Phytologist xx, xx
DOI: http://dx.doi.org/10.1111/nph.14070.

Resource Description

Title: High-resolution community profiling of arbuscular mycorrhizal fungi
FOR816dw ID: 1493
Publication Date: 2016-07-06
License and Usage Rights: PAK 823-825 data user agreement. (www.tropicalmountainforest.org/dataagreementp3.do) http://www.tropicalmountainforest.org/dataagreement.do
Resource Owner(s):
Individual: Klaus Schlaeppi
Contact:
Individual: S. Franz Bender
Contact:
Individual: Fabio Mascher
Contact:
Individual: Giancarlo Russo
Contact:
Individual: Andrea Patrignani
Contact:
Individual: Tessa Camenzind
Contact:
Individual: Stefan Hempel
Contact:
Individual: Matthias C. Rillig
Contact:
Individual: Marcel G.A. van der Heijden
Contact:
Abstract:
Summary
Community analyses of arbuscular mycorrhizal fungi (AMF) using ribosomal small subunit
(SSU) or internal transcribed spacer (ITS) DNA sequences often suffer from low resolution or
coverage. We developed a novel sequencing based approach for a highly resolving and specific profiling of AMF communities.
We took advantage of previously established AMF-specific PCR primers that amplify a
c. 1.5-kb long fragment covering parts of SSU, ITS and parts of the large ribosomal subunit
(LSU), and we sequenced the resulting amplicons with single molecule real-time (SMRT)
sequencing.
The method was applicable to soil and root samples, detected all major AMF families and
successfully discriminated closely related AMF species, which would not be discernible using
SSU sequences. In inoculation tests we could trace the introduced AMF inoculum at the
molecular level. One of the introduced strains almost replaced the local strain(s), revealing
that AMF inoculation can have a profound impact on the native community.
The methodology presented offers researchers a powerful new tool for AMF community
analysis because it unifies improved specificity and enhanced resolution, whereas the drawback of medium sequencing throughput appears of lesser importance for low-diversity groups
such as AMF.
Keywords:
| arbuscular mycorrhiza | mycorrhiza |
Literature type specific fields:
ARTICLE
Journal: New Phytologist
Volume: xx
Page Range: xx
Metadata Provider:
Individual: Tessa Camenzind
Contact:
Online Distribution:
Download File: http://www.tropicalmountainforest.org/publications.do?citid=1493

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